This document describes the steps involved in using a magnetic bead to separate proteins.
This guide provides step-by-step instructions for preparing, washing, and eluting samples using the Agilent Multiple Affinity Removal Spin Cartridge. It covers dilution, filtration, buffer exchange, and fraction collection for mouse serum. The cartridge is designed for proteomics research and can be re-equilibrated for reuse. Additional products for proteomics are listed on Agilent’s website.
The part number is 5188-5289.
Dilute 25-30-µL mouse serum sample to 200 µL with Buffer A.
Centrifuge 1.5 min at 100 x g.
Fill new 5-ml plastic syringe with 4-ml Buffer A.