This document provides a guide on how to use the Agilent Technologies Agilent MassHunter Workstation Software – Data Acquisition for 6410 Triple Quad LC/MS. It covers system preparation, data acquisition preparation, development of acquisition methods, determination of precursor ion masses, finding optimum fragmentor voltage, determination of product ion masses, and finding optimum collision energy for MRM acquisition. Users can learn to use the Agilent 6410 Triple Quad LC/MS instrument and optimize instrument parameters for best sensitivity through these exercises.
This guide walks you through setting up and optimizing data acquisition for the Agilent 6410 Triple Quad LC/MS. It includes tasks for configuring LC and MS parameters, determining precursor and fragmentor voltages, and finding optimal collision energies for MRM transitions. The guide uses sample data files and step-by-step instructions to help users understand how to maximize sensitivity and use the Qualitative Analysis program effectively.
The guide assumes you have installed the Data Acquisition software and configured your instrument. It covers preparing your system, setting up sample data, and performing method development using a worklist approach. It also references other Agilent resources like the Installation Guide, Quick Start Guide, and Concepts Guide for additional support.
Set the flowrate to 800 pL/min, Solvent A to 5 mM NH4HCO3 in H2O, Solvent B to 5 mM NH4HCO3 in ACN, and use the gradient: 0 min - 13%, 1.80 min - 60%, 2.50 min - 60%, Stop Time 2.50 min, Post Time 2.50 min.
Use ESI (positive polarity) as the source, Gas Temp 350 °C, Nebulizer 50 psi, Dry Gas 12 L/min, Range 100 to 400, and Scan Type MS2Scan.
Sulfamethizole: 271.0 > 155.8, Fragmentor 100V, Collision 10; Sulfamethazine: 279.0 > 185.7, Fragmentor 100, Collision 15; Sulfachloropyridazine: 285.0 > 155.7, Fragmentor 100, Collision 10; Sulfadimethoxine: 311.0 > 155.7, Fragmentor 100, Collision 20.