This manual provides instructions on how to prepare the Gene Expression Wash Buffer 1 and 2 with Triton X-102 detergent.
This guide explains how to add Triton X-102 detergent to Agilent Gene Expression Wash Buffers 1 and 2 to reduce background artifacts. The step-by-step procedure includes opening the cubitainer, adding 2 mL of 10% Triton X-102, mixing, and labeling. The addition is mandatory for miRNA profiling and optional for gene expression profiling. The guide also includes safety and usage notes, including calibration of pipettes and restrictions on resale or diagnostic use.
Yes, adding Triton X-102 is mandatory for miRNA profiling applications.
No, adding Triton X-102 is optional for gene expression applications.
Add 250 μL of 10% Triton X-102 to achieve a final concentration of 0.005%.