This manual introduces the operation of the Agilent 1100 series dual-loop autosampler PS, guiding users to use the device correctly and obtain the best recovery results.
This guide explains how to optimize recovery results using the Agilent 1100 Series dual-loop autosampler PS. It covers key settings like rinse and flush solvents, sample draw and eject speeds, and loop fill factors. The push-through design requires special attention to solvent compatibility and injection parameters to avoid precipitation and ensure accurate results.
The guide includes practical recommendations such as using a 50:50 DMSO/acetonitrile mixture for viscous solvents, limiting draw speed to 20000 µL/min, and lowering eject speed for better recovery. It also provides a chart showing the effect of eject speed on recovery and a graph illustrating peak area linearity with partial loop fill.
Use a solvent in which the sample is readily soluble, such as acetonitrile, methanol, or DMSO/acetonitrile 50:50 v/v.
The rinse volume should be at least 300 µL, calculated from the injection volume and a user-defined factor.
The sample draw speed should not exceed 20000 µL/min for best recovery results.
Lower eject speeds yield better recoveries; the default 10000 µL/min should be lowered further.