This manual describes the protocol for using HaloPlex Target Enrichment System for Illumina Sequencing
This document describes an optimized protocol for using the Agilent HaloPlex target enrichment system. It details the necessary reagents and equipment required before starting an experiment, including specific kits from Agilent and compatible third-party supplies like AMPure XP beads.
The guide outlines a ten-step workflow to prepare target-enriched sequencing libraries. Key steps include digesting genomic DNA with restriction enzymes, hybridizing digested DNA to HaloPlex probes, capturing the target DNA, ligating fragments, removing non-target DNA via Haloase digestion, and amplifying/indexing the captured libraries.
| Samples per run configuration | 12 samples |
|---|---|
| Recommended genomic DNA amount | 800 ng plus 100 ng excess |
| Total genomic DNA required | 900 ng |
The protocol is optimized for digestion of 800 ng of genomic DNA plus 100 ng excess DNA, totaling 900 ng.
No, this system is for research use only and is not intended for use in diagnostic procedures.
Samples may be stored at –20°C, but they must not undergo multiple freeze/thaw cycles.