This kit can be used to detect mouse tumor necrosis factor soluble receptor I (TNFsR-I). It adopts double antibody sandwich ELISA. The sensitivity can reach 0.5 ng/mL, and the detection range is 0.5-50 ng/mL.
The kit is intended for research use only and employs a double‑antibody sandwich ELISA to measure mouse tumor necrosis factor soluble receptor I (TNF‑sR‑I). The document describes sample collection (serum, plasma, cell supernatant, tissue homogenate), required equipment, and detailed assay steps including reagent preparation, plate washing, incubation, and optical density reading at 450 nm.
Performance data are provided, showing high accuracy (R ≥ 0.9900), sensitivity (LOD < 10 pg/mL), specificity (no cross‑reaction), and repeatability (CV < 15%). Storage conditions, shelf life, and safety notices are also included. This manual is written in Chinese.
| Accuracy | R ≥ 0.9900 |
|---|---|
| Sensitivity (LOD) | < 10 pg/mL |
| Specificity | No cross‑reaction with similar soluble structures |
| Repeatability | CV < 15 % |
| Storage Temperature | 2‑8 °C, protected from light |
| Shelf Life | 6 months |
No, the kit is for research use only and must not be used for clinical or diagnostic purposes.
Store the kit at 2 °C to 8 °C, protected from light and moisture.
The assay can detect concentrations lower than 10 pg/mL.
Repeated freeze‑thaw cycles should be avoided; aliquot reagents and store at –20 °C if not used immediately.