The 17-hydroxycorticosteroid (17-OHCS) ELISA kit is designed by the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) principle, which is used to detect the content of 17-hydroxycorticosteroid (17-OHCS) in human serum, plasma, cell supernatant and tissue homogenate.
The kit uses a double‑antibody sandwich ELISA to detect mouse tumor necrosis factor soluble receptor‑1 (TNFSR‑1). Samples, standards and HRP‑labeled detection antibody are added to pre‑coated microplates, incubated, washed, and developed with TMB substrate. The resulting color change is measured at 450 nm and compared to a standard curve to determine concentration.
The manual details sample collection (serum, plasma, cell supernatant, tissue homogenate), storage conditions (‑20 °C or ‑80 °C, avoid freeze‑thaw), required equipment (microplate reader, precision pipettes, 37 °C incubator), reagent preparation, step‑by‑step assay procedure, and data analysis. Precautions, specifications, and a disclaimer for research‑only use are also provided. This manual is written in Chinese.
| Detection Limit | <10 pg/mL |
|---|---|
| Accuracy (R²) | ≥0.9900 |
| Repeatability (CV) | <15 % |
| Storage Temperature | 2‑8 °C |
| Shelf Life | 6 months |
The assay can detect concentrations lower than 10 pg/mL.
Reagents should be stored at 2‑8 °C and allowed to equilibrate to room temperature for 20 minutes before use.