Rat GST ELISA Kit User Manual

About this manual

This kit is designed to measure glutathione S‑transferase (GST) levels in rat serum, plasma or related liquid samples using an indirect ELISA method. Purified rat GST antibody coats the microplate, and samples are compared against known standards to generate a calibration curve.

The document lists all kit components, sample requirements (e.g., no NaN₃), detailed assay steps, washing and incubation conditions, and important precautions such as storage, avoiding repeated freeze‑thaw cycles, and proper waste disposal. This manual is written in Chinese.

What's inside

• Kit Overview
• Principle of the Assay
• Reagent List
• Sample Requirements
• Assay Procedure
• Washing and Incubation Steps
• Result Calculation
• Precautions
• Specifications
• Storage and Shelf Life

Specifications

Frequently asked questions

Can samples containing NaN₃ be tested with this kit?

No, samples that contain NaN₃ cannot be tested because NaN₃ inhibits the activity of HRP.

How should the kit be stored before use?

The kit should be kept refrigerated and allowed to equilibrate at room temperature for 15‑30 minutes before use; unopened plates should be resealed in a sealed bag after opening.

What is the detection range of the assay?

The assay can detect GST concentrations from 50 to 1500 mIU/mL.