Rat retinol binding protein (RBP) ELISA kit user manual, for research use only. It is expected to quantitatively measure RBP in rat serum, plasma or other related liquids by ELISA.
This manual describes how to prepare standards, reagents, and samples, and how to perform the ELISA assay for rat retinol‑binding protein (RBP). It includes detailed protocols for plate loading, incubation, washing, substrate development, and optical density measurement.
Additional sections cover storage conditions for reagents, precautions to avoid hemolysis, manual and automated plate‑washing methods, and guidance on data interpretation using a 450 nm wavelength reader. This manual is written in Chinese.
| Microplate format | 96‑well |
|---|---|
| Reading wavelength | 450 nm |
| Sample volume per well | 100 µL |
| Detection substrate | TMB (3,3',5,5'-Tetramethylbenzidine) |
The optical density is measured at 450 nm.
The kit uses a 96‑well microplate.
100 µL of sample or reagent is added to each well.