Key facts
- Manuals
- 10
- Categories
- 1
- Languages
- ZH
- Last added
- 2012-03-28
Featured Elisa manuals
Thermo Scientific Mouse TNF-sR-I ELISA Kit User Manual
The kit is intended for research use only and employs a double‑antibody sandwich ELISA to measure mouse tumor necrosis factor soluble receptor I (TNF‑sR‑I). The document describes sample collection (serum, plasma, cell supernatant, tissue homogenate), required equipment, and detailed assay steps including reagent preparation, plate washing, incubation, and optical density reading at 450 nm.
Performance data are provided, showing high accuracy (R ≥ 0.9900), sensitivity (LOD < 10 pg/mL), specificity (no cross‑reaction), and repeatability (CV < 15%). Storage conditions, shelf life, and safety notices are also included. This manual is written in Chinese.
Covers: Principle of Detection · Sample Collection and Storage · Required Equipment · Precautions · Reagent Kit Composition · Reagent Preparation
TNFSR-1 ELISA Kit User Manual
The kit uses a double‑antibody sandwich ELISA to detect mouse tumor necrosis factor soluble receptor‑1 (TNFSR‑1). Samples, standards and HRP‑labeled detection antibody are added to pre‑coated microplates, incubated, washed, and developed with TMB substrate. The resulting color change is measured at 450 nm and compared to a standard curve to determine concentration.
The manual details sample collection (serum, plasma, cell supernatant, tissue homogenate), storage conditions (‑20 °C or ‑80 °C, avoid freeze‑thaw), required equipment (microplate reader, precision pipettes, 37 °C incubator), reagent preparation, step‑by‑step assay procedure, and data analysis. Precautions, specifications, and a disclaimer for research‑only use are also provided. This manual is written in Chinese.
Covers: Detection Principle · Sample Collection and Storage · Materials Required · Reagent Preparation · Assay Procedure · Result Calculation
E1850r Rat GAG ELISA Kit User Manual
This manual describes the intended applications of the rat GAG ELISA kit, which include quantitative measurement of GAG in rat serum, plasma, tissue homogenates, cell‑culture supernatants, and other related liquids. It explains the sandwich ELISA principle, reagent composition, and detailed preparation of standards and detection solutions.
The document provides a complete assay workflow: sample handling, plate loading, incubation times, washing procedures (both manual and automated), substrate development, termination, and optical density reading at 450 nm. It also covers calculation of sample concentrations, specificity notes, detection range, storage conditions, and shelf‑life information. This manual is written in Chinese.
Covers: Intended Application · Principle of Assay · Reagent Composition and Preparation · Sample Preparation · Assay Procedure · Plate Washing Methods
Rat Retinol Binding Protein (RBP) ELISA Kit User Manual
This manual describes how to prepare standards, reagents, and samples, and how to perform the ELISA assay for rat retinol‑binding protein (RBP). It includes detailed protocols for plate loading, incubation, washing, substrate development, and optical density measurement.
Additional sections cover storage conditions for reagents, precautions to avoid hemolysis, manual and automated plate‑washing methods, and guidance on data interpretation using a 450 nm wavelength reader. This manual is written in Chinese.
Covers: Reagent and Standard Preparation · Assay Procedure · Plate Washing Methods · Precautions and Tips · Optical Density Measurement · Reagent Storage
Latest Elisa manuals
Envirologix transgenosis ELISA kit operating instructions
This document provides information on the testing procedure of the EnviroLogix GMO Plate Kit, including sample preparation, addition of reagents, mixing and incubation, and result interpretation.
Thermo Scientific Mouse TNF-sR-I ELISA Kit User Manual
User Manual 6 pages · 220 KB · ZHThis manual provides step‑by‑step instructions for using the Thermo Scientific mouse TNF‑sR‑I ELISA kit in research, covering sample handling, assay…
TNFSR-1 ELISA Kit User Manual
User Manual 6 pages · 220 KB · ZHInstructions for using the TNFSR-1 mouse ELISA kit, covering principle, sample handling, assay steps, and result calculation.
E1850r Rat GAG ELISA Kit User Manual
User Manual 3 pages · 98 KB · ZHThe E1850r rat GAG ELISA kit user manual provides step‑by‑step instructions, reagent preparation, assay procedure, and data analysis for measuring GAG in…
Rat Retinol Binding Protein (RBP) ELISA Kit User Manual
User Manual 5 pages · 109 KB · ZHStep‑by‑step instructions, reagent preparation, and assay procedures for the rat RBP ELISA kit.
E0928m Mouse p53 ELISA Kit Manual
User Manual 2 pages · 90 KB · ZHThe E0928m mouse p53 ELISA kit manual provides instructions, reagent composition, and assay procedures for measuring p53 in mouse samples.
IL-6 ELISA Kit User Manual
User Manual 2 pages · 163 KB · ZHInstructions for using the human IL-6 ELISA kit to measure IL-6 levels in serum, plasma and other biological fluids.
melamine (Melamine)ELISA quick test kit Manual
Rat GST ELISA Kit User Manual
User Manual 2 pages · 168 KB · ZHThe manual provides step‑by‑step instructions for using the rat GST ELISA kit, covering principle, reagents, sample handling, assay procedure, and safety notes.
rat Multi- Gather ADP ribose Polymerization Enzyme (PARP) elisa immunoassay (ELISA) kit Manual
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Elisa manuals
Can this ELISA kit be used for clinical diagnosis?
No, the kit is for research use only and must not be used for clinical or diagnostic purposes.
From: Thermo Scientific Mouse TNF-sR-I ELISA Kit User Manual
What temperature should the kit be stored at?
Store the kit at 2 °C to 8 °C, protected from light and moisture.
From: Thermo Scientific Mouse TNF-sR-I ELISA Kit User Manual
What is the detection limit of the kit?
The assay can detect concentrations lower than 10 pg/mL.
At what temperature should the kit be kept before use?
Reagents should be stored at 2‑8 °C and allowed to equilibrate to room temperature for 20 minutes before use.
What types of samples can be measured with this kit?
The kit can quantify GAG in rat serum, plasma, tissue homogenate, cell‑culture supernatant, or other related liquids.
How is the standard solution prepared?
Dilute the 100 ng/mL standard stock with sample diluent to create a series of standards (100, 50, 25, 12.5, 6.25, 3.12, 1.56 ng/mL) and use 0 ng/mL as the blank.