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GENSCRIPT Human Recombinant EP2 Prostanoid Receptor Stable Cell Line Version 10132010 user manual
This manual provides an overview of the human recombinant EP2 prostanoid receptor stable cell line (CHO‑K1/EP2/Ga15) supplied by GenScript. It includes background information on EP2 signaling, representative calcium‑mobilization data, and detailed thawing and subculturing procedures.
The document also contains a limited‑use license agreement and references to scientific literature supporting the cell line’s applications in functional assays for the EP2 receptor.
GenScript CHO-K1 specifications
| Quantity | Two vials of frozen cells (3x 10° per vial) |
|---|---|
| Stability | 16 passages |
| Application | Functional assay for EP2 receptor |
| Freeze Medium | 45% culture medium, 45% FBS, 10% DMSO |
| Complete Growth Medium | Ham’s F12, 10% FBS |
| Culture Medium | Ham’s F12, 10% FBS, 400 µg/ml G418, 100 µg/ml Hygromycin B |
| Mycoplasma Status | Negative |
| Storage | Liquid nitrogen immediately upon delivery |
Source: GENSCRIPT Human Recombinant EP2 Prostanoid Receptor Stable Cell Line Version 10132010 user manual
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Questions answered in these manuals
How many vials are supplied with the cell line?
Two vials of frozen cells are supplied, each containing approximately 3 × 10⁶ cells.
From: GENSCRIPT Human Recombinant EP2 Prostanoid Receptor Stable Cell Line Version 10132010 user manual
What is the stability of the CHO‑K1/EP2/Ga15 cell line?
The cell line remains stable for up to 16 passages.
From: GENSCRIPT Human Recombinant EP2 Prostanoid Receptor Stable Cell Line Version 10132010 user manual
What antibiotic concentrations are required for selection?
Add Hygromycin B to 200 µg/ml and G418 to 400 µg/ml the day after seeding.
What is the stability of the cell line?
The cell line remains stable for up to 16 passages.
How should the CHO-K1/ADRB2/Ga15 cell line be thawed?
Remove the vial from liquid nitrogen, thaw quickly in a 37 °C water‑bath, decontaminate the outside with 70 % ethanol, transfer to a tube with complete growth medium, centrifuge at 200 × g for 5 min, discard the medium and resuspend the cells in fresh complete growth medium.
From: GenScript CHO-K1/ADRB2/Ga15 Cell Line Technical Manual
What subcultivation ratio is recommended for this cell line?
A subcultivation ratio of 1:3 to 1:8 is recommended weekly.
From: GenScript CHO-K1/ADRB2/Ga15 Cell Line Technical Manual