Agilent Manuals (Industrial)

This Application Note shows three system configurations for recovery collection with the Agilent 1100 Series purification system1,2. One configuration uses a 12-position/13-port valve 3 in the waste line of the fraction collector, which offers up to twelve recovery locations. Another possibility is to use a third-party fraction collector, controlled by the ChemStation through external contacts using a BCD board. The most sophisticated approach, however, is to use a dedicated Agilent 1100 Series fraction collector, which gives complete recovery location tracking with the graphical user interface of the software or in the reports.

File format: PDF Size:492 KB

This document describes a GC/MS method using RTL technology that allows for the analysis of 567 of the most common pesticides and endocrine disrupters used worldwide. By utilizing the RTL functionality with the 6890/5973 GC/MSD system, a new database containing mass spectra and compound retention times has been developed. When combined with the compound screening tools in the MSD Productivity ChemStation software, data files can be easily, accurately, and quickly screened for the presence of any of the 567 compounds listed in the database. Additionally, the software features enable the detection of compounds that may usually be masked by others.

File format: PDF Size:5250 KB

The four antiarrhythmic drugs, quinidine, disopyramide, procainamide and its metabolite N-acetylprocainamide are separated by HPLC using gradient analysis on a reversed phase column and UV detection. The method is accurate and reliable, and can be used for the determination of these drugs in biological fluids.

File format: PDF Size:27 KB

The document describes a drug called Verapamil, which is a calcium antagonist used to treat hypertension, cardiac arrhythmia, and angina pectoris. The drug also shows some prophylactic effects against migraines.

File format: PDF Size:34 KB

The HPLC method presented here shows the chromatogram of the four common antiasthmatic drugs theophylline, theobromine, caffeine and emprofylline using isocratic analysis on a reversed phase column and UV detection.

File format: PDF Size:29 KB

This document introduces the detailed steps for analyzing testosterone and its metabolites using HPLC.

File format: PDF Size:43 KB

This document describes the collection of fractions from an oligonucleotide separation using gel electrophoresis and offline MALDI-TOF analysis. By using the Agilent oligonucleotide kit and Agilent Capillary Electrophoresis system, antisense oligonucleotides can be successfully separated. The Agilent ChemStation software allows for fully automated collection of fractions using signal A as the peak sensor. This method enables further analysis of the separated peaks and determination of the molecular mass.

File format: PDF Size:66 KB

This article describes the use of high-performance liquid chromatography (HPLC) for the analysis of fat-soluble vitamins. HPLC can separate and detect these compounds at room temperature while blocking oxygen and light. UV-visible diode-array detection provides both qualitative and quantitative data. Electrochemical detection is also a highly sensitive and selective HPLC method. Different food matrices require different extraction procedures, including alkaline hydrolysis, enzymatic hydrolysis, alcoholysis, direct solvent extraction, and supercritical fluid extraction.

File format: PDF Size:143 KB

File format: PDF Size:0 KB

Orthogonal methods for protein purification and purity analysis are important to determine with certainty protein identity and purity. The Agilent 1100 Series purification system and the Agilent 2100 bioanalyzer with the Protein 200 Plus LabChip® kit provide an ideal solution that ensures efficient and reliable protein purification and characterization.

File format: PDF Size:225 KB

This article introduces the Statistical Peak Identification feature in Agilent Technologies' Cerity NDS for Chemical QA/QC, which addresses the complications in traditional peak identification algorithms caused by peak shifts, changes in concentration, and external forces. It provides a more robust quantitation.

File format: PDF Size:189 KB

This application note describes the methods for measuring protein size and protein expression using the Agilent 2100 Bioanalyzer and Protein 200 LabChip® kit.

File format: PDF Size:234 KB

This application note describes how to use microbore reversed-phase HPLC to separate peptide mixtures, to characterize proteins or to isolate peptides for further studies.

File format: PDF Size:224 KB

This application note describes reproducible methods for sample preparation and HPLC analysis of PNAs in soil, barbecued meat and waste water, all with good recovery rates, and detection limits that exceed regulatory requirements.

File format: PDF Size:259 KB

This note provides a method for improving GC performance in order to perform trace level GC/MS analysis. The note summarizes suggestions for improving GC performance, including using high purity carrier gas, optimizing sample injection and using optimized GC columns.

File format: PDF Size:359 KB

This document describes the characteristics of gas chromatograph-mass spectrometer, including electronic pressure control, fast GC furnace, etc., which makes gas chromatograph-mass spectrometer have extremely high productivity, and it can realize small volume sample injection and automatic data processing.

File format: PDF Size:487 KB

The document describes the ability to identify and quantify compounds based on single and dual column analyses. Instruments used include Agilent Technologies 6890 with other Hewlett-Packard instruments and data systems. The document demonstrates the acquisition and analysis of combined GC/MSD and GC/FID data.

File format: PDF Size:418 KB

File format: PDF Size:0 KB

This article introduces the method of optimizing determination of C1-C6 impurities in propylene and ethylene using HP-PLOT/Al2O3 columns. Compared with other commercially available alumina PLOT columns, HP-PLOT columns have better selectivity, increased capacity and complete baseline separation. HP-PLOT column analyses were done using the 5890 Series II gas chromatograph with electronic pressure control and a flame ionization detector.

File format: PDF Size:147 KB

This Application Note demonstrates mass-based purification of a series of synthetic peptides with the Agilent 1100 Series purification system. The modular set-up of the system, a reliable fully-automated delay volume calibration and a comprehensive software package provide a versatile platform for purification tasks up to 100 mL/min. Crude peptides were successfully purified by reverse phase HPLC from less than 1 kDa to more than 10 kDa, covering a pI range from 4 to 13.

File format: PDF Size:325 KB