Agilent Manuals (Industrial)

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This application note describes how the Agilent 2100 bioanalyzer can be used for the detection of a point mutation in the human prothrombin gene. The prothrombin protein plays a key role in blood clotting. A single nucleotide polymorphism (SNP), i.e. a point mutation in the prothrombin gene results in a common hereditary predisposition to venous thrombosis. We present a convenient and reliable mutation detection method by a PCR-based assay including a specific restriction digest (PCR-RFLP). Due to its high sensitivity and resolution, the applied microfluidic assay proves to be extremely suitable for detec- tion of the obtained PCR products and restriction fragments. The developed method is suitable for the workflow at Laborigo molecular diagnostic laboratory.

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The 5975 inert MSD – Benefits of Enhancements in Chemical Ionization Operation Technical Note This Technical Note describes the complete integration of chemical ionization (CI) on the 5975 inert MSD (mass selective detector), which now makes all the power of CI mass spectrometry (MS) conveniently available. CI has been fully automated by providing auto-tuning with electronic control of the reagent gases. All modes of MS operation in a single sequence, completely free of operator intervention, are now possible. Analytes can be sensitively determined in samples by implementing alternating positive- or negative-CI (PCI or NCI) modes. On the 5975 inert MSD, CI is as easy as EI (electron impact ionization).

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This article presents a method for quantitatively analyzing opiates in urine using liquid chromatography/mass selective detector time-of-flight (LC/MSD TOF). The method utilizes accurate mass measurement to enhance selectivity.

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This document describes the analysis method for the pharmaceutical compound clozapine, used for the treatment of severely ill schizophrenic clients. The use of Zorbax SPE C18 EC cartridge significantly reduces levels of interfering substances in the bloodstream, allowing for accurate quantification of clozapine or clozapine-like compounds.

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This application note describes a method for the analysis of volatile organic compounds (VOCs) in water samples according to US Environmental Protection Agency (USEPA) method 8260B.

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This article introduces the new features of Agilent Technologies, Inc.'s 5973N inert mass spectrometer detector and ChemStation software, which allows for operation of the ion source at higher temperatures, providing improvements in analysis. It also provides advice and examples on how to explore the utility of ion source temperature.

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A USGS analytical method using gas chromatography/mass selective detector (GC/MSD) was modified and used to identify the source of pesticides and to calculate the pesticide load in river water during storm seasons. The method was validated to cover 12 organophosphate pesticides and 5 pyrithroids. The limits of quantitation for these target compounds are 10–500 ppt using a GC/MSD selected ion monitoring (SIM) method.

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The use of a highly inert capillary column can facilitate the quantitation of low levels of very active compounds. The use of the highly inert HP-5MSi column in conjunction with the Agilent 6890/5973 inert gas chromatograph/mass spectrometer (GC/MS) system allows the measurement of pesticides and environmental contaminants at low levels while maintaining response linearity over a wide concentration range.

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The document introduces a method for determining residual solvents in pharmaceuticals using Agilent G1888 Network Headspace Sampler.

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This document introduces the optimized sources for electron ionization (EI) and chemical ionization (CI) of Agilent 5973x gas chromatograph/mass selective detectors (GC/MSDs). It also demonstrates how to acquire high-quality EI spectra with the CI source without changing the sources. The experimental results show that using the CI source can obtain comparable major and minor peaks to the EI source.

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This document introduces a GC/MS with ECD method for determination of isosorbide-5-mononitrate in human plasma.

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Laser Ablation (LA)-ICP-MS for Production Control of Nickel Alloys

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This study describes the development of a robust high-throughput analytical method for the determination of 18 elements (15 trace elements and 3 electrolytes) in blood and serum samples using an Agilent Technologies 7500ce collision/reaction cell ICP-MS system. The only sample preparation necessary was dilution using an alkaline diluent containing ammonia, EDTA Triton X-100, and butan-1-ol. Instrument calibration was performed using external calibration with internal standardization. The performance of the method exceeded a previously-used magnetic sector HR-ICP-MS method by at least a factor of three in terms of sample throughput and matched the precision and detection limits of that method.

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The Agilent 7500c Inductively Coupled Plasma Mass Spectrometer (ICP-MS) was used for the fast, routine, and reliable analysis of 23 trace elements in urine.

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This application note demonstrates the suitability of a newly developed, high sensitivity reaction cell inductively coupled plasma mass spectrometer (ICP-MS) for the determination of inorganic impurities in semiconductor grade sulfuric acid. The Agilent 7500cs ICP-MS, featuring a high sensitivity version of the Octopole Reaction System (ORS), was used to analyze sulfuric acid for all the metals required by the semiconductor industry. The enhanced ORS eliminates all plasma and matrix based polyatomics that interfere with the measurement of elements such as K, Ca, Ti, Cr, Fe, Co, Ni, Cu, and Zn while offering excellent ion transmission and sensitivity. Furthermore, all analytes can be measured at high plasma power to promote complete decomposition of the sulfuric acid in the plasma. This leads to high signal stability and a reduced frequency of routine maintenance. Sample preparation is a simple 10x dilution followed by direct analysis thereby significantly reducing the potential for the sample contamination frequently encountered with evaporative matrix elimination techniques.

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This application note demonstrates the suitability of a newly developed, high sensitivity reaction cell inductively coupled plasma mass spectrometer (ICP-MS) for the deter- mination of inorganic impurities in semiconductor-grade tetramethyl ammonium hydroxide (TMAH).

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Inductively coupled plasma mass spectrometry can be used as a powerful screening tool for the presence of toxic elements and chemicals in the environment. It has the ability to quickly and quantitatively screen many types of samples for toxic metals at ultratrace levels. When hyphenated with gas or liquid chromatography systems, it can also distinguish toxic from nontoxic species of most elements including radioactive forms. Using retention time locking and compound independent calibration, gas chromatography inductively coupled plasma mass spectrometry (GC-ICP-MS) has the potential to accurately identify and quantify a wide range of volatile toxic organic compounds without the necessity of possessing or analyzing standards for each target analyte. As such, when used in conjunction with other analytical techniques, inductively coupled plasma mass spectrometry can be a valuable tool in the arsenal against environmental terrorism.

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A new technique for the analysis of corundum that has undergone heat treatment and beryllium diffusion to resemble one of the most valuable colored stones: vivid orange-yellow gems known as padparadscha is developed in this study. The technique is based on laser-induced breakdown spectroscopy (LIBS), and chemical analysis with inductively coupled plasma-mass spectrometry (ICP-MS) is used to verify the LIBS data. The method can be used to distinguish natural and treated gems, especially in the case of unknown treatments. This study shows that the method can be used to determine the authenticity of gems.

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