Protocol describing Agilent's SureSelect Paired-End Target Enrichment System steps for preparing DNA libraries for Illumina sequencing.
This guide provides Agilent's recommended procedures for capturing genomic regions of interest using the SureSelect Paired‑End Target Enrichment System kit. It covers safety, required reagents and equipment, and detailed steps for sample preparation, hybridization, and post‑hybridization amplification.
The document is organized into four main sections: (1) Before You Begin – safety notes and procedural guidelines; (2) Sample Preparation – DNA shearing, end repair, adapter ligation, and size selection; (3) Hybridization – library capture on magnetic beads; and (4) Post‑Hybridization Amplification – PCR amplification, purification, and quality assessment with the Agilent 2100 Bioanalyzer.