This is a document about Uni-ZAP XR Vector Kit and Uni-ZAP XR Gigapack Cloning Kits EcoR I/Xho I/CIAP-Treated Instruction Manual
This document provides instructions for using the Uni-ZAP XR Vector Kit and Uni-ZAP XR Gigapack Cloning Kits, designed for research use only. It covers materials provided, storage conditions, bacterial host strains, helper phage handling, and protocols for ligating inserts, packaging reactions, and screening. The kits include EcoR I/Xho I-digested vectors, helper phages, and packaging extracts for lambda library construction with blue-white selection. Special handling is required for sensitive packaging extracts and helper phages to maintain efficiency.
The manual includes detailed protocols for excision, troubleshooting, and storage, along with references to Agilent’s technical support and website for further assistance.
| Vector | EcoR I and Xho I digested, CIAP treated |
|---|---|
| Storage Temperature - Vector | -20°C |
| Storage Temperature - Helper Phage | -80°C |
Store the vector at -20°C. After thawing, aliquot and store at -20°C. Do not pass through more than two freeze-thaw cycles.
Store the helper phage at -80°C.