Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
This document is a technical manual for GenScript Protein G Resin (Cat. No. L00209), an affinity chromatography medium designed for the one-step purification of immunoglobulins. The resin features a recombinant protein G ligand coupled to 4% highly cross-linked agarose, offering a static binding capacity greater than 20 mg sheep IgG/ml settled resin. It is specifically optimized for purifying mammalian monoclonal and polyclonal IgGs that may not bind well to Protein A, including human IgG3, mouse IgG1, and rat IgG2a.
| Ligand | Recombinant Streptococcal protein G |
|---|---|
| Matrix | Spherical 4% cross-linked agarose |
| Average particle size | 90 μm (45-165 μm) |
| Storage solution | 1X PBS containing 20% ethanol |
| Static binding capacity | > 20 mg sheep IgG/ml settled resin |
| Number of IgG binding sites per ligand | 3 |
Store the resin at 2-8 °C in 1X PBS containing 20% ethanol.
Yes, columns can be regenerated up to 10 times without significant loss of binding capacity.
De-gas buffers and samples, and do not allow the column to dry.