Key facts
- Brand
- GenScript
- Model
- L00209
- Category
- Health Care
- Documents
- 3
- Languages
- EN
- Last added
- 2026-10-14
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GenScript Protein G Resin L00209 Technical Manual
This document is a technical manual for GenScript Protein G Resin (Cat. No. L00209), an affinity chromatography medium designed for the one-step purification of immunoglobulins. The resin features a recombinant protein G ligand coupled to 4% highly cross-linked agarose, offering a static binding capacity greater than 20 mg sheep IgG/ml settled resin. It is specifically optimized for purifying mammalian monoclonal and polyclonal IgGs that may not bind well to Protein A, including human IgG3, mouse IgG1, and rat IgG2a.
Read onlineGenScript L00209 specifications
| Matrix | spherical agarose, 4% |
|---|---|
| Average particle size | 90 μm (45–165 μm) |
| Static binding capacity | >20 mg sheep IgG/ml drained medium |
| Storage condition | 20% ethanol at +4 to +8 °C |
| Number of IgG binding sites per ligand | 3 |
| MW of ligand | Approximately 22 kDa |
| pI of ligand | 4.69 |
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Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
Which IgG subclasses does Protein G Resin bind to?
It has greater affinity for human IgG3, mouse IgG1, and rat IgG2a.
Does Protein G Resin bind to human IgM, IgD, or IgA?
No, unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.
What is the recommended storage temperature for Protein G Resin?
Store the resin at 2-8 °C in 1X PBS containing 20% ethanol.
Can the column be regenerated?
Yes, columns can be regenerated up to 10 times without significant loss of binding capacity.
What is the static binding capacity of Protein G Resin?
The static binding capacity is greater than 20 mg goat IgG/ml settled resin.
How should buffers be prepared for use?
Water and chemicals should be of high purity, and buffers should be filtered through a 0.45 µm filter before use.