GenScript One-Step WesternTM Blot Kit User Manual

GenScript One-Step WesternTM Blot Kit is a breakthrough immuno-detection technology (patent pending) for fast Western blot and Dot blot.


GenScript One-Step WesternTM Blot Kit User Manual - cover page
Brand
GenScript
Category
Health Care
Document type
User Manual
Language
English
Pages
5
File format
PDF
File size
185 KB
Published
18 October, 2023
Updated
18 October, 2023
MD5 checksum
637ACC76DB554DC884BF9C402AD0AB2F

About this manual

This manual describes the GenScript One-Step WesternTM Blot Kit, designed for rapid Western and Dot blot detection using a simplified one-hour protocol. It replaces the traditional three-step method with a single incubation step, reducing time and optimization needs. The kit includes reagents for rabbit or mouse primary antibodies and is compatible with GenScript’s proprietary membranes and substrates for optimal results. Storage is at 4°C, and components should not be frozen.

What's inside

  • Description
  • Kit Contents
  • Applications
  • Key Features
  • Storage
  • One-Step Western Protocol
  • Examples Using the Kit
  • Troubleshooting
  • Order Information

Specifications

Detection Time1 hour
Storage Condition4°C (stable for 6 months)
Detection ChemistryHorse radish peroxidase (HRP) based

Frequently asked questions

How long does the One-Step WesternTM Blot Kit take to complete?

The kit allows you to perform Western blot or Dot blot within an hour.

How long does the One-Step WesternTM Blot Kit procedure take?

It allows you to perform Western blot or Dot blot within an hour.

What are the two kit catalog numbers for rabbit and mouse primary antibodies?

L00204 is for rabbit primary antibody, and L00205 is for mouse primary antibody.

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