This is a manual for Sheep MAN1A1 ELISA Kit. It describes the assay principle, kit components, assay planning, experimental layout, sample collection, sample preparation, standard preparation, reagent preparation, assay procedure, calculation of results, troubleshooting guide, assay usage and support.
This document is the user manual for the LSBio Sheep MAN1A1 ELISA Kit (Catalog No. LS-F45522), designed for the detection of the MAN1A1 target in sheep samples. The kit utilizes a sandwich ELISA principle involving a pre-coated microtiter plate, biotin-conjugated detection antibodies, and an Avidin-Horseradish Peroxidase (HRP) conjugate to generate a colorimetric signal measured at 450 nm.
| Target | MAN1A1 |
|---|---|
| Species | Sheep |
| Detection Method | Colorimetric - 450nm |
| Assay Principle | Sandwich ELISA |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Assay Diluent B Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Detection Reagent B Volume | 1 vial x 120 µl |
| Wash Buffer Volume | 1 vial x 20 ml |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
| Plate Sealers Quantity | 4 |
| Storage Temperature (Short term) | 4°C |
| Storage Temperature (Long term) | -20°C |
| Usage Limit | 1 month |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The optical density (OD) of the well is measured at a wavelength of 450 nm ± 2 nm.
The kit should be stored at 4°C if intended for use within 24 hours, or at -20°C for longer storage of specific components.
Required equipment includes a microplate reader with a 450nm wavelength filter, high-precision pipette, sterile pipette tips, Eppendorf tubes, and a 37°C incubator.