This is a manual for Bovine ANXA3 / Annexin A3 ELISAKit (Sandwich ELISA)
This document is the user manual for the LSFbio LS-F14321 Bovine ANXA3 / Annexin A3 ELISA Kit, which utilizes the Sandwich ELISA principle. The kit is intended for research use only and is not approved for diagnostic use in humans or clinical diagnosis. It includes detailed instructions on assay planning, sample collection and preparation, reagent preparation, and the step-by-step assay procedure.
| Target | Bovine ANXA3 / Annexin A3 |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials (Lyophilized) |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Assay Diluent B Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Detection Reagent B Volume | 1 vial x 120 µl |
| Wash Buffer Quantity | 1 vial x 20 ml (30 x) |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
| Plate Sealers Quantity | 4 |
| Required Equipment | Microplate reader with 450nm wavelength filter |
| Incubator Temperature | 37 °C |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The optical density (OD) of the well is measured at a wavelength of 450 nm ± 2 nm.
Check pipettes for accuracy, ensure proper standard dilution by spinning and mixing, and completely aspirate wells between steps.