This is a product manual that introduces the use of the Rat OIT3 ELISAKit (Sandwich ELISA) product produced by LSBio.
This document is the user manual for the LSFbio LS-F18383 Rat OIT3 ELISA Kit, which utilizes the sandwich ELISA assay principle for the detection of OIT3 in rat samples. The kit is intended for research use only and is not approved for diagnostic use in humans or clinical diagnosis.
The manual provides comprehensive instructions on assay planning, sample collection and preparation for various sample types (e.g., plasma, serum, cell lysates), and the detailed experimental procedure. It includes information on kit components, storage conditions, calculation of results, and a troubleshooting guide for common issues such as poor standard curves or high background.
| Detection Method | Colorimetric - 450nm |
|---|---|
| Target Species | Rat |
| Kit Components | Coated 96-well Strip Plate, Standards, Diluents, Detection Reagents, Wash Buffer, TMB Substrate, Stop Solution |
| Storage Temperature | 4 °C or -20 °C |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The optical density (OD) of the well is measured at a wavelength of 450 nm ± 2 nm.
The kit should be stored at 4 °C if intended for use within 24 hours.