This is a sample manual of ELISA kit.
This document provides the user manual for the LSF357 Human LGMN/Legumain ELISA Kit, which utilizes the Sandwich ELISA principle for the detection of Legumain. The kit is intended for research use only and is not approved for diagnostic use in humans or clinical diagnosis.
The manual outlines the complete workflow, including kit components, storage requirements, and necessary supplies. It details the experimental layout, sample collection and preparation methods for various biological samples, and the step-by-step assay procedure. Additionally, it includes instructions for calculating results and a troubleshooting guide to address common issues such as poor standard curves or high background.
| Detection Method | Colorimetric - 450nm |
|---|---|
| Wavelength | 450 nm ± 2 nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Wash Buffer Quantity | 1 vial x 20 ml (30 x) |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The assay is based on the Sandwich ELISA principle where a capture antibody binds the target antigen, followed by a biotin-conjugated detection antibody and an Avidin-HRP conjugate.
Required equipment includes a microplate reader with a 450nm filter, high-precision pipettes, sterile tips, Eppendorf tubes, a 37 ° C incubator, and deionized water.