This document introduces Agilent MassHunter Workstation for identifying unknown compounds using TIC analysis and deconvolution methods.
The document serves as a familiarization guide for the Agilent MassHunter Workstation software, specifically focusing on the Unknowns Analysis application. It is designed to teach users how to identify compounds through specific analytical techniques.
The guide presents step-by-step exercises that can be performed using demonstration data files shipped with the system or user-acquired data. The content covers three main tasks: identifying compounds using Total Ion Chromatogram (TIC) analysis, identifying compounds using deconvolution, and generating final reports.
The guide covers identifying compounds using TIC Analysis, which uses integration instead of deconvolution, and Deconvolution, which extracts clean spectra based on retention time and peak shape.
If the default layout is not present, click View > Preset Layout > Standard to restore it before creating a new analysis.