This technical note describes how to achieve high throughput and high resolution liquid chromatography analysis using columns with internal diameters of less than or equal to 2.1 mm. By optimizing column length, flow rate, gradient, and detector, run times of less than 30 seconds can be achieved.
This document serves as a technical note detailing methods to optimize the Agilent 1100 Series HPLC system for higher sample throughput when using columns with internal diameters of 1 and 2 mm. It addresses the trade-off between high throughput and the limitations of narrow-bore columns, such as long run times and equilibration periods. The text outlines specific strategies to reduce cycle times while maintaining precision and resolution.
| Column internal diameter | 1 and 2 mm |
|---|---|
| Recommended flow rate for 1-mm id columns | 0.01 to 0.05 ml/min |
| System delay volume reduction | Upchurch low delay volume mixer |
The limiting factors include the ability of the detector to collect sufficient data points for high precision and low limits of detection, as well as backpressure and equilibration times.
The reduction of column length is the most important parameter to shorten cycle times.