Introduces the design of the Agilent 1200 Series column compartment and the influence of the new design on dispersion and detector noise.
This document describes the Agilent 1200 Series column compartment SL, which features a new design allowing operation over an enhanced temperature range up to 100 °C. It includes a post-column cooling device designed to reduce detector noise and improve the limit of detection at high flow rates and elevated temperatures.
The note details the influence of low-delay volume heaters and coolers on peak dispersion and resolution. It demonstrates that the new low-delay volume pre-column heater (1.6 pL) reduces dispersion compared to standard heaters, while the post-column cooler (1.5 pL) maintains a high signal-to-noise ratio by cooling the column effluent to the detector temperature.
| Column temperature range | 10 degrees below ambient up to 100 °C |
|---|---|
| Flow rate range | 50 pL up to 5 mL/min |
| Pre-column heater delay volume | 1.6 pL |
| Post-column cooler delay volume | 1.5 pL |
The column compartment supports temperatures up to 100 °C.
It reduces the noise level generated in the detector, which improves the limit of detection and quantification.