This document compares the Agilent Quick Amp and Low RNA Input Linear Amplification Kits and shows that both kits are equivalent when used for gene expression profiling.
This document is a technical note comparing the performance of Agilent's Quick Amp Labeling Kits and the older Low RNA Input Linear Amplification Kits (LRILAK). The study utilized MicroArray Quality Control (MAQC) consortium RNA samples to evaluate one-color and two-color gene expression experiments. Results indicated that while both kits are functionally equivalent, the Quick Amp kits demonstrated superior reliability with zero labeling failures, attributed to ISO 13485 compliant manufacturing and minor reformulations. The document advises researchers to use only one kit type within a single study to avoid subtle variations affecting data interpretation.
The study was designed to demonstrate that the Quick Amp Labeling kit and the LRILAK kit provide equivalent functionality.
Data extraction was performed using Agilent Whole Human Genome applications software, while Minitab 15, GeneSpring GX 9.0, and JMP 6 were used for statistical analyses.
Researchers are encouraged not to use both kits within the same study because subtle variations may lessen the ability to observe small differences in gene expression.