The Agilent Automated Card Extraction (AACE) LC/MS System is a fully integrated instrument for the automated analysis of Dried Blood Spots (DBS) and other dried media. The system consists of the AACE instrument which enables automated flow-through analysis of DBS cards, together with software to control online analyte extraction, trapping, and LC/MS analysis. The AACE instrument is a CTC-based autosampler platform which integrates with the Agilent 1200 Infinity Series HPLC to provide analytical LC separation. Quantitative analysis is performed using any of the Agilent 6400 Series Triple Quadrupole LC/MS Systems. Data processing and reporting is performed using MassHunter software.