The document introduces Agilent's RNA assay instrument, which can measure total RNA, mRNA and Cy5-labeled RNA, and estimate their concentration and quality within minutes.
The Agilent RNA 6000 Nano LabChip kit is designed for the characterization of total RNA and mRNA samples, as well as the estimation of their concentrations. It is compatible with the Agilent 2100 bioanalyzer and utilizes RNase-free reagents and chips to ensure sample integrity during analysis.
| Quantitative range (Total RNA) | 25-500 ng/µL |
|---|---|
| Qualitative range (Total RNA) | 5-500 ng/µL |
| Maximum sample buffer strength | < 10 mM TE |
| Analysis run time | 30 minutes |
| Number of samples per chip | 12 samples/chip |
| Sample volume | 1 µL |
The kit allows analysis using as little as 200 pg of total RNA.
The automated analysis of 11 or 12 samples takes about 30 minutes.
Indications include a decreasing ratio of ribosomal bands, additional peaks below ribosomal bands, a decrease in overall RNA signal, and a shift towards shorter fragments.