Agilent Zorbax 300Extend-C18 HPLC Column Datasheet

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Agilent Zorbax 300Extend-C18 HPLC Column Datasheet - cover page
Brand
Agilent
Document type
Datasheet
Language
English
Pages
4
File format
PDF
File size
152 KB
Published
04 July, 2012
Updated
30 September, 2023
MD5 checksum
9C7027A8943745233908E90D72BE169F

About this manual

The Agilent Zorbax 300Extend-C18 is a reversed-phase HPLC packing designed for stable use with high pH mobile phases. It incorporates a patented bidentate organosilane combined with double end-capping to protect the ultra-pure Type B silica support from dissolution at high pH levels.

This column is particularly well-suited for separating highly basic compounds as free bases, offering excellent stability and chromatographic separation properties across low, intermediate, and high pH ranges. The uniform spherical particles are based on a specially prepared Zorbax 300Rx-SIL porous silica microsphere support with a nominal surface area of 50 m²/g.

What's inside

  • General Description
  • Column Characteristics
  • Safety Considerations
  • pH Guidelines
  • Instrument Guidelines
  • Operational Guidelines
  • Mobile Phase Selection
  • Applications

Specifications

Silica Support TypeUltra-pure Type B silica
Nominal Surface Area50 m²/g
Pore Size300 Angstroms
Carbon Contentabout 4%
Maximum Operating Temperature60°C
Optimum Operating pH RangepH 2 - 11.5

Frequently asked questions

What is the maximum pH limit for this column?

The column has been used up to pH 11.5 with good results, provided certain operating parameters are followed.

Which organic buffers are recommended for high pH applications?

Organic buffers such as pyrrolidine, triethylamine, and 1-methyl-piperidine are recommended because carbonate and phosphate buffers should be avoided.

What is the preferred organic modifier for high pH applications?

Methanol is a preferred organic modifier because the rate of silica support dissolution is slower with this solvent than with acetonitrile.

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