The document describes the features and characteristics of a product in detail, including its functions and performance. The product is made of high-quality materials and has a sleek and simple design. It is easy to operate and provides stable, reliable performance with a long lifespan. It is suitable for various scenarios and needs, making it an ideal choice.
The document describes the Agilent Zorbax SAX, a polar bonded-phase column packing designed for anion exchange high performance liquid chromatography (HPLC). The packing consists of ammonium functionalized silane chemically bonded to Zorbax SIL particles through Si-O-Si bonds. It is manufactured using a trifunctional organo silane reagent to maximize stability with aqueous mobile phases and ensure uniform spherical shape for optimum column efficiency.
The datasheet provides detailed operational guidelines, including safety conditions regarding toxic or flammable mobile phases and the respirable nature of dry Zorbax packings. It outlines operating parameters such as maximum pressure (400 bar) and temperature (40°C), along with recommendations for mobile phase selection, pH range (2 to 7.0), and the use of guard columns. The document also covers column care procedures, including filtration requirements, backflushing methods, frit replacement instructions, and storage recommendations in pure organic solvents.
| Particle Type | ammonium functionalized silane chemically bonded to Zorbax SIL particles |
|---|---|
| Bonding Mechanism | Si-O-Si bonds |
| Maximum Operating Pressure (4.6 and 9.4 mm ID) | 400 bar (6000 psi) |
| Maximum Operating Temperature | 40°C |
| Recommended pH Range | 2 to 7.0 |
| Inlet Frit Porosity | nominal porosity of 2 um |
Long term storage should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
No, users should avoid use of this column below pH 2.0 or above pH 7.0.
The maximum operating pressure for the 4.6 and 9.4 mm ID columns is 400 bar (6000 psi).