This document is about the Huawei nova8Pro smartphone user manual, which mainly introduces the functions, operating methods and precautions of the phone.
The Agilent ZORBAX Bio Series OligoColumn is specifically developed for the separation and purification of single-stranded oligonucleotides, including heterodeoxyoligonucleotides with chain lengths ranging from 3 to 48 bases. The column is designed for both analytical and preparative chromatography applications, capable of handling sample loads of 200-300 micrograms per run with minimal loss in resolution or purity. Oligonucleotides isolated using this column typically achieve purities well in excess of 90-95%.
| Chain Length Range | 3 to 48 bases |
|---|---|
| Analytical Column Dimensions | 6.2mm ID x 80mm |
| Sample Load Capacity | 200-300 micrograms |
| Typical Purity | 90-95% |
| Maximum Operating Pressure | 300 bar |
| Maximum pH Limit | 8.5 |
| Minimum pH Limit | 2.5 |
Acetonitrile is the most useful organic solvent for the system, as substituting methanol or tetrahydrofuran has a negative effect on resolution and efficiency.
Yes, the column may be used for the separation of oligoribonucleotides in addition to oligonucleotides.
A 90-minute gradient from 35% to 80% B (where B is Solvent 1 + 1.0 M NaCl) is recommended as a good first run.