This video introduces the use and precautions of autosamplers and injectors for the Agilent 1260 and 1290 LC system.
This document provides troubleshooting guidance for Agilent Liquid Chromatography (LC) autosamplers and injection methods. It features insights from Applications Chemist Mike Woodman and Senior Applications Scientist Bill Long, focusing on common operational issues.
The two main types of autosampler design are fixed loop and variable volume.
Most newer instruments, including the Agilent 1260 and Agilent 1290, have variable volume injectors.
To validate autosampler accuracy, install a tared vial and inject a volume that is conveniently verified gravimetrically.
Extra peaks may be caused by contamination in the needle port or needle seat from other samples, or from the mobile phase.