Agilent Bio IEX columns are designed for use with high pressure liquid chromatography (HPLC) systems. Loose fittings and connections can cause buffer leaks and sample loss. Many samples and buffers are considered hazardous and should be treated as such. Always wear gloves and safety glasses when using columns in an HPLC system. Agilent does not recommend opening HPLC columns for any reason.
Agilent Bio IEX columns are packed with polymeric, nonporous, ion exchange particles designed for high-resolution, high-recovery, and highly efficient separations of proteins, peptides, oligonucleotides, and other bio-molecules. The columns feature highly cross-linked and rigid nonporous poly(styrene divinylbenzene) particles grafted with a hydrophilic layer to eliminate nonspecific binding.
The datasheet provides detailed technical specifications including particle sizes (1.7, 3, 5, and 10 µm), dynamic binding capacities for various phases (SCX, WCX, SAX, WAX), and operating limits such as pH stability (2-12), temperature (up to 80 °C), and pressure ratings for both stainless steel and PEEK hardware.
| Particle Size | 1.7, 3, 5 and 10 um |
|---|---|
| Pore Structure | Nonporous |
| pH Stability | 2-12 |
| Operating Temperature Limit | 80 °C |
| Hardware Pressure Limit (Stainless Steel) | 600 bar (8,700 psi) |
| Hardware Pressure Limit (PEEK) | 400 bar (5,800 psi) |
| Working Flow Rate | 0.1-1.0 mL/min |
They are designed for high-resolution separations of proteins, peptides, oligonucleotides, and other bio-molecules.
No, SCX and WCX columns are not compatible with cationic detergents.
The recommended cleaning buffer is 50 mM phosphate buffer in 1.0 M NaCl, pH 10.