Agilent Technologies has developed and optimized products and solutions to address an entire range of protein identification challenges. Let our comprehensive line of protein identification tools put you on the road to faster discovery and identification of low- abundance proteins within complex samples.
Agilent Technologies offers an integrated workflow for protein identification that addresses the challenge of identifying low-abundance proteins within complex biological samples. The solution combines optimized preparation products with outstanding separation technologies and sensitive mass spectrometry performance.
The document details specific Agilent solutions across four main steps: sample preparation using Multiple Affinity Removal systems and fractionation columns; sample separation via HPLC-Chip/MS or Nanoflow-LC systems; sample analysis using ion trap MS tools; and sample identification. It highlights how these integrated technologies enable the identification of up to 10-fold more proteins compared to non-depleted samples.
| Protein recovery rate | >98% |
|---|---|
| Increase in identified proteins | up to 10-fold |
| Flow rate range | 100 nl/min to 1 pl/min |
A single sample can contain thousands of proteins with abundances varying over many orders of magnitude, often making low-abundance proteins difficult to detect.
This column recovers more than 98% of proteins and peptides from immunodepleted human, mouse, and rat serum samples.
The number of identified proteins in the sample can increase as much as 10-fold.
The EFC system provides stability and reliability at flow rates spanning 100 nl/min to 1 pl/min.