The document introduces Agilent's Whole Human Genome Oligo Microarray Kit with SurePrint Technology. This product is designed to provide researchers with a solution for whole human genome microarrays. It utilizes a 1" x 3" (25mm x 75mm) glass slide microarray format and offers comprehensive coverage. The microarray contains highly-sensitive 60-mer oligo probes that can detect low expressing genes. The content of the microarray has been validated through experiments. The product offers flexibility and sensitivity in microarray experiments.
The document provides technical specifications for the Agilent Whole Human Genome Oligo Microarray Kit (Catalog 60-mer), which includes five microarrays. The kit utilizes a 1" x 3" glass slide format with approximately 41,000 human genes and transcripts covered by experimentally validated 60-mer oligonucleotide probes.
| Number of microarrays in kit | 5 |
|---|---|
| Number of genes and transcripts | ~41,000 |
| Feature size | 115 ym |
| Microarray format | AAK |
| Oligo probe length | 60-mer |
| Probe orientation | Sense |
| Median SD | < 0.013 |
| Detection sensitivity | 1 transcript/cell/million cells* |
| Dynamic range | Over 3 orders of magnitude |
The overall assay time is 18.5 hours.
The kit uses Cyanine 3 & cyanine 5 fluorescent labeling.