Agilent's novel and powerful method of location analysis of DNA binding proteins pairs chromatin immunoprecipitation (ChIP) with Agilent DNA microarrays to allow the construction of comprehensive DNA-protein binding profiles. This oligonucleotide microarray enables you to use ChIP-on-chip technology to gain broad insight into the mechanisms of human gene regulation on a genome-wide scale.
The document introduces the Agilent Human Promoter Arrays and ChIP-on-Chip Set as a method for location analysis of DNA-binding proteins. It combines chromatin immunoprecipitation (ChIP) with Agilent DNA microarrays to construct comprehensive DNA-protein binding profiles on a genome-wide scale.
Key features include proprietary SurePrint technology using 60-mer oligonucleotide probes and a two-color labeling system for high sensitivity and accuracy. The set covers approximately 17,000 human transcripts with probe coverage from -5.5 KB upstream to +2.5 KB downstream of transcriptional start sites.
The model number is G4489A.
There are approximately 25 probes per transcript.
The set covers -5.5 KB upstream to +2.5 KB downstream from the transcriptional start sites.