The document introduces a novel dried matrix adsorbent for extracting acidic drugs from blood samples. By optimizing the desorption conditions, the adsorbent can enhance the sensitivity of detecting acidic drugs.
The document investigates variables affecting the efficiency of Dried Matrix Spotting (DMS) or Dried Blood Spotting (DBS), a microsampling technique for pharmaceutical development. It specifically focuses on improving the sensitivity analysis of acidic analytes, such as statins (atorvastatin, simvastatin, pravastatin, and lovastatin).
The study utilizes Agilent Bond Elut DMS cards, which feature a novel noncellulose-based substrate to reduce nonspecific binding. The text details experiments comparing various desorption solvents (methanol and acetonitrile with different modifiers) and methods (centrifugation versus soaking). Results indicate that 60% methanol with 1% ammonium hydroxide is often the optimal solvent for overall sensitivity across multiple statins, while specific conditions vary by compound.
Methanol at 60% with 1% ammonium hydroxide appeared to be the optimal desorption solvent for atorvastatin, simvastatin, pravastatin, and lovastatin.
Atorvastatin could be detected easily at a concentration of 1 ng/mL.
The total run time for the separation and analysis is 8 minutes.