This Application Note describes the transfer of a United States Pharmacopeia (USP) method for prednisolone from normal phase HPLC to SFC using the Agilent 1260 Infinity Hybrid SFC/UHPLC System. The results show that the SFC method meets the system suitability criteria, is 4x faster, and results in 17x lower solvent expenses. Robustness tests on the SFC method demonstrate excellent robustness for routine analysis.
The document details a method transfer for analyzing prednisolone, replacing a toxic and expensive normal phase HPLC method with a faster, eco-friendly SFC method. The study utilizes the Agilent 1260 Infinity Hybrid SFC/UHPLC System to perform both methods on a single instrument, eliminating system variability and saving costs.
The SFC flow rate is maintained at 2.9 mL/min.
The SFC run time is 5.5 minutes.