This document describes the GC analysis of total fatty acid methyl esters (FAME) and methyl linolenate in biodiesel using the revised EN14103:2011 method. Four different types of biodiesel samples were manually prepared and analyzed, and the total FAME and methyl linolenate contents were determined with precision exceeding the method’s specifications.
This document details the operation and performance of the Agilent 7890 Series Gas Chromatograph (GC) when configured to run the revised European method EN14103:2011. The method is used to determine the total Fatty Acid Methyl Ester (FAME) content and methyl linolenate content in finished B100 biodiesel. The note specifically addresses improvements in precision and chromatographic performance for samples containing animal fats and mixed feedstocks.
| Column | 19091N-133 HP-INNOWax Column, 30m x 0.25 mm, 0.25 pm |
|---|---|
| Split flow | 100 mL/min |
| Carrier gas | Helium at 1 mL/min constant flow |
| Initial Column Temperature | 60 °C for 2 min |
| Ramp 1 | 10°C /min to 200 °C |
| Ramp 2 | 5 °C/min to 240 °C |
| Final Hold | Hold 240 °C for 7 min |
The document uses the revised EN14103:2011 method for determining FAME content in biodiesel.
The Agilent 7890 Series GC is configured to run the EN14103:2011 method.
Methyl nonadecanoate (C19:0) is used as the internal standard.