Agilent Small RNA Kit is a reagent kit for small RNA analysis, including Small RNA Chips, Small RNA Dye Concentrate, Small RNA Marker, Small RNA Conditioning Solution Syringe Kit, Small RNA Gel Matrix, Small RNA Ladder etc.
This guide provides step-by-step instructions for preparing and using the Agilent Small RNA Kit for small RNA fragment analysis. It covers equipment setup, reagent storage and handling, sample preparation, and loading protocols. The kit is designed for use with the Agilent 2100 bioanalyzer and includes chips, dyes, and reagents for electrophoretic separation of nucleic acids.
Key safety and handling notes include protecting dyes from light, using RNase-free materials, and heating RNA samples before use. The guide also includes protocols for preparing gel-dye mix, loading samples, and running assays with specific timing and temperature requirements.
| Analysis run time | 30 minutes |
|---|---|
| Analysis range | 6-150 nt |
| Number of samples | 11 samples/chip |
| Quantitation reproducibility | 25 % CV (for ladder as sample) |
| Sample volume | 1 ul |
| Quantitative range | 50-2000 pg/l for purified miRNA |
| Kit stability | 4 months (Storage temperature see individual box!) |
| Sensitivity (Signal/Noise > 3) | 50 pg/ul (for 40 nt fragment of diluted ladder) |
Freeze the unused Small RNA Ladder at -20 °C and keep all reagents and reagent mixes refrigerated at 4°C when not in use.
The recommended concentration for total RNA is 1-100 ng/ul, and for purified small RNA samples (< 150 nt) is 1-20 ng/ul.
The gel-dye mix should be used within 4 weeks after preparation.