provides an introduction to Agilent ChemStation for UV-visible Spectroscopy software
This handbook details the operation of the kinetics and thermal denaturation modes within the biochemical analysis software of the Agilent ChemStation for UV-visible spectroscopy. It describes how to acquire time-based and temperature-based data using either a manual sampling system or a multicell transport system.
The guide explains the processes of data transformation and the specific calculations used in evaluating experimental results, such as initial rates, zero order kinetics, first-order reactions, and melting temperatures for DNA melts. The manual is designed to help users follow good laboratory practice (GLP) guidelines.
| Software Revision Support | B.02.xx revisions |
|---|---|
| Manual Sampling Wavelengths | up to six wavelengths |
| Multicell Transport Samples | up to eight samples |
| Integration Time Range | 0.1-26.5 seconds |
The Kinetics mode supports a Manual Sampling System for single cells and a Multicell Transport System for multiple samples.
No, measurements are restricted to a single wavelength which is the same for each cell.