This manual provides guidelines for optimizing the performance of the Pursuit™XRs Ultra 2.8 and Pursuit™UPS2.4 HPLC columns for high speed separations. The manual provides information on reducing system dead-volume and other band broadening contributions to achieve maximum separation efficiency with these low-volume columns.
The document outlines specific requirements for the HPLC system to achieve high efficiency with small particle size columns, such as minimizing dead volume in connecting tubing and detector flow cells. It details best practices for injector design, sample solvent preparation, and injection volumes to prevent band broadening.
Technical specifications are provided for two column series: the Pursuit UPS 24 supports a maximum pressure of up to 400 bar (5800 psi), while the Pursuit XRs 2.8 supports up to 1034 bar (15,000 psi).
| Maximum Pressure (Pursuit UPS 24) | up to 400 bar/5800 psi |
|---|---|
| Maximum Pressure (Pursuit XRs 2.8) | up to 1034 bar/15,000 psi |
Use narrow internal diameters of at most 0.010-inch (0.25 mm) to minimize dead volume.
The detector flow cells must have a small internal volume, preferably less than 2 uL.