The document is the Hi-Plex column installation and maintenance instructions, which introduces the connection and installation, transportation liquid, column conditioning and other contents of the Hi-Plex column.
This document provides operational guidance for the installation and connection of Hi-Plex ligand exchange columns. It details proper tightening procedures for end fittings and specifies requirements for shipping eluent handling to prevent column drying.
The guide covers mobile phase preparation, sample filtration, and routine maintenance protocols including efficiency testing and column clean-up procedures. It also outlines system shutdown steps and long-term storage conditions.
Nuts should be tightened finger tight and then 1/4 turn using a wrench.
The mobile phase should be run at 0.1 mL/min during initial conditioning.
Yes, they may be stored in a refrigerator at 4 °C but must not freeze.