EcoSpheres are spherical beads of a microporous, chemically neutral copolymer of styrene and divinylbenzene of a controlled particle size.
EcoSpheres are spherical beads made of a microporous copolymer used for separating low molecular weight organic species from high molecular weight components via gel permeation chromatography. The document details the necessary steps to prepare these beads, including swelling them in appropriate organic solvents before use.
The guide provides specific instructions for assembling and packing EcoSpheres into glass or stainless steel columns. It covers procedures such as creating a uniform slurry, pouring it into the column, and establishing flow from bottom to top to ensure proper bed formation and prevent air pockets.
| Maximum flow rate | 10 mL/min |
|---|---|
| Maximum back pressure | 1 MPa |
| Recommended flow rate range | 5 and 10 mL/min |
| Minimum swelling time | 6 hours |
No, highly polar solvents such as methanol or water should not be used because no swelling will occur.
The material must be removed from the column, re-swelled, and the column re-packed.