This document is the user guide of PL-WCX & PL-SCX HPLC Columns, which mainly introduces the function, installation and use method as well as maintenance and maintenance of the product, such as column connection, tube connection, etc.
This document provides installation guidelines for Agilent PL-WCX (weak cation) and PL-SCX (strong cation) exchange media. It details the use of compatible stainless steel tubing and fittings, specifically noting that Parker or Vici Valeo nuts are recommended to ensure proper sealing and avoid leaks.
| Maximum Operating Pressure | 3000 psi (207 bar) |
|---|---|
| Operating Temperature | up to 80 °C |
| pH Range Stability | 1-13 |
| Maximum Flow Rate (4.6 mm ID) | 4.0 mL/min |
| Warranty Period | 60 days |
1/16 in. stainless steel tubing is recommended, with specific inner diameters (ID) required based on the work type.
Parker compatible 1/16 in. nuts and ferrules are recommended, while fittings from Waters and Rheodyne may not be compatible.
Wash out shipping solution, exchange counter ion by eluting with high ionic strength buffer until baseline stabilizes, then equilibrate with low ionic strength buffer.
Flush with water, then introduce a storage buffer of approximately 0.1 M Na2SO4 containing 0.2% sodium azide.