This document is an instruction manual for SatisFection Transfection Reagent. It provides information on the usage, storage conditions, and additional materials required for this product. The transfection reagent is for research use only and not intended for diagnostic procedures. The product comes with a limited warranty for product replacement and Agilent disclaims any other warranties. The document also includes ordering information and technical support contact details.
The document serves as an instructional guide for the SatisFection Transfection Reagent, a cationic polymer designed to optimize gene expression and protein production in common, hard-to-transfect, and primary cell lines. It details the reagent's mechanism of action, which involves binding DNA into a polyplex that enters cells via endocytosis while protecting it from degradation.
The manual outlines specific pre-protocol considerations such as DNA quality requirements, optimal cell density (60-80% confluence), and recommended reagent-to-DNA ratios. It provides detailed step-by-step procedures for transfecting adherent cells in various tissue culture formats, including volume guidelines for 6-well to 100-mm dishes, instructions for preparing the transfection mixture, and protocols for both transient and stable transfections.
| Catalog Numbers | #204121, #204122, and #204123 |
|---|---|
| Storage Temperature | 4°C |
| Recommended DNA Concentration per Well (24-well) | 0.6 ug |
| Optimal Cell Density at Transfection | 60-80% confluence |
| Recommended Reagent to DNA Ratio (Starting Point) | 1.5 ul reagent per ug DNA |
The polymer binds and condenses DNA into a polyplex that enters the cell via endocytosis, facilitating endosomal rupture and protecting DNA from degradation.
Maximum gene expression and protein production are observed 36-72 hours post-transfection.
No, a media change is not required prior to adding the transfection mixture; the medium may contain serum or be serum-free.