TKB1 Competent Cells is a plasmid expression vector for DNA transformation experiments. This instruction manual provides the characteristics, usage methods and precautions for this product.
The document details the use of Agilent TKB1 competent cells, a tyrosine kinase derivative of the BL21(DE3) strain designed for protein expression. It outlines the materials provided in the kit, necessary reagents, and specific storage requirements at -80°C to maintain efficiency.
| Transformation Efficiency | >5 x 10^5 cfu/μg of pUC18 DNA |
|---|---|
| Storage Temperature | -80°C |
| Recommended Storage Duration | 6 months |
| Optimal Heat Pulse Time | 45–50 seconds |
Competent cells must be stored at -80°C to prevent a loss of efficiency.
Cells stored directly from the dry ice shipping container at -80°C should retain their guaranteed efficiency for 6 months.
The optimal heat pulse duration is between 45 and 50 seconds.