Use Agilent One-Color Microarray-Based Exon Analysis Low Input Quick Amp WT Labeling Protocol for gene expression analysis
This document describes recommended procedures for preparing and labeling complex biological targets, hybridizing, washing, scanning, and extracting features from Agilent 60-mer oligonucleotide microarrays. It is specifically designed for use with the Low Input Quick Amp WT Labeling kit and Agilent Gene Expression Exon microarrays.
| Starting input requirement (2-pack, 4-pack, 8-pack microarrays) | 25 ng of total RNA |
|---|---|
| Starting input requirement (1-pack microarray) | 100 ng of total RNA |
| Recommended Specific Activity for hybridization | 15 pmol cyanine 3 per ug cRNA |
Users should generally follow Biosafety Level 1 (BL1) safety rules.
The starting input requirement is 25 ng of total RNA.
The input requirement is 100 ng of total RNA.
The recommended Specific Activity is increased to 15 pmol cyanine 3 per ug cRNA.