SureSelect Illumina Paired-End mRNA-Seq Library Prep DNA Capture for Multiplexed Sequencing Protocol is a protocol for SureSelect RNA Capture for Illumina Paired-End Multiplexed Sequencing
This guide details the steps to prepare and hybridize samples for target enrichment using Biotinylated RNA oligomer libraries (Bait) on the Illumina paired-end multiplex sequencing platform. The document covers procedures from mRNA purification through library amplification, index tag addition, and final quality assessment.
The protocol is specifically optimized for use with Agilent SurePrint Technology and includes detailed instructions for sample preparation, hybridization capture, post-hybridization amplification to add index tags, and pooling samples for multiplexed sequencing. It also lists required reagents, equipment, and safety notices.
No, the SureSelect platform is for research use only and not for use in diagnostic procedures.
In general, follow Biosafety Level 1 (BL1) safety rules when working with the reagents.
The Biotinylated RNA oligomer libraries are used to enrich targeted regions of the transcriptome.