Agilent Technologies Vitality hrGFP Subcellular Localization Vectors Instruction Manual

This document introduces the features and characteristics of Agilent's Vitality hrGFP Subcellular Localization Vectors phrGFP-Nuc, phrGFP-Mito, phrGFP-Golgi, and phrGFP-Perox.

Brand: Agilent
Document type: User Manual
Language: English
Pages: 17
File format: PDF
File size: 324 KB
Published: 06 June, 2012
Updated: 30 September, 2023
MD5 checksum: 32E2AE41F31584687153C1362209B2A4
PDF: Agilent Technologies Vitality hrGFP Subcellular Localization Vectors Instruction Manual PDF

About this manual

The document describes the Agilent Technologies Vitality hrGFP Subcellular Localization Vectors, which include phrGFP-Nuc, phrGFP-Mito, phrGFP-Golgi, and phrGFP-Perox. These vectors are designed for non-commercial research use only to facilitate real-time monitoring of specific organelles in mammalian cells.

What's inside

Materials Provided
Storage Conditions
CMV Promoter
Non-Commercial Research Use License
Description of the Vectors
Cre-Mediated Recombination Protocol
Confirming Recombination
Mammalian Cell Transfection
Specifications for hrGFP and EGFP Excitation and Emission Spectra
Troubleshooting

Specifications

Concentration	1.0 pg/pl
Quantity per catalog item	20 ug
Storage Temperature	-20°C

Frequently asked questions

What is the intended use of these vectors?

The products are licensed for non-commercial research purposes only and must not be used in diagnostic procedures.

Which organelles can be monitored using these vectors?

The vectors allow monitoring of peroxisomes, mitochondria, Golgi, and nuclei.

What is the excitation peak for hrGFP?

The major excitation peak for the cloned GFP protein is at 500 nm.

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